rabbit polyclonal antibody against phosphorylated igf-1 receptor β subunit Search Results


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(a) Brn3c:GFP marked hair cells (outlined) immunolabeled for phosphorylated <t>IGF1R</t> receptor. Scale = 10µm. (b-c) Mean pIGF1R immunofluorescence normalized to GFP immunofluorescence (b) and mean number of pIGF1R puncta (c) per hair cell cluster. *p<0.05, unpaired t test, Welch-corrected. N= 8 larvae, 1-3 neuromasts/larva. (d-f) Mean percentage of surviving hair cells 4 hours post-neomycin treatment. To calculate hair cell survival percentage, hair cell counts in neomycin treated larvae were normalized to vehicle treated larvae of same genotype. (d) Wildtype larvae treated with the IGF1R antagonist NVP-AEW541. (e-f) pappaa p170 larvae treated with SC79 (e) or recombinant <t>IGF1</t> (f). * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. One-way ANOVA, Holm-Sidak post test. N=10 larvae, 3 neuromasts/larva. Error bars=SEM.
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(a) Brn3c:GFP marked hair cells (outlined) immunolabeled for phosphorylated <t>IGF1R</t> receptor. Scale = 10µm. (b-c) Mean pIGF1R immunofluorescence normalized to GFP immunofluorescence (b) and mean number of pIGF1R puncta (c) per hair cell cluster. *p<0.05, unpaired t test, Welch-corrected. N= 8 larvae, 1-3 neuromasts/larva. (d-f) Mean percentage of surviving hair cells 4 hours post-neomycin treatment. To calculate hair cell survival percentage, hair cell counts in neomycin treated larvae were normalized to vehicle treated larvae of same genotype. (d) Wildtype larvae treated with the IGF1R antagonist NVP-AEW541. (e-f) pappaa p170 larvae treated with SC79 (e) or recombinant <t>IGF1</t> (f). * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. One-way ANOVA, Holm-Sidak post test. N=10 larvae, 3 neuromasts/larva. Error bars=SEM.
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(a) Brn3c:GFP marked hair cells (outlined) immunolabeled for phosphorylated <t>IGF1R</t> receptor. Scale = 10µm. (b-c) Mean pIGF1R immunofluorescence normalized to GFP immunofluorescence (b) and mean number of pIGF1R puncta (c) per hair cell cluster. *p<0.05, unpaired t test, Welch-corrected. N= 8 larvae, 1-3 neuromasts/larva. (d-f) Mean percentage of surviving hair cells 4 hours post-neomycin treatment. To calculate hair cell survival percentage, hair cell counts in neomycin treated larvae were normalized to vehicle treated larvae of same genotype. (d) Wildtype larvae treated with the IGF1R antagonist NVP-AEW541. (e-f) pappaa p170 larvae treated with SC79 (e) or recombinant <t>IGF1</t> (f). * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. One-way ANOVA, Holm-Sidak post test. N=10 larvae, 3 neuromasts/larva. Error bars=SEM.
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(a) Brn3c:GFP marked hair cells (outlined) immunolabeled for phosphorylated <t>IGF1R</t> receptor. Scale = 10µm. (b-c) Mean pIGF1R immunofluorescence normalized to GFP immunofluorescence (b) and mean number of pIGF1R puncta (c) per hair cell cluster. *p<0.05, unpaired t test, Welch-corrected. N= 8 larvae, 1-3 neuromasts/larva. (d-f) Mean percentage of surviving hair cells 4 hours post-neomycin treatment. To calculate hair cell survival percentage, hair cell counts in neomycin treated larvae were normalized to vehicle treated larvae of same genotype. (d) Wildtype larvae treated with the IGF1R antagonist NVP-AEW541. (e-f) pappaa p170 larvae treated with SC79 (e) or recombinant <t>IGF1</t> (f). * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. One-way ANOVA, Holm-Sidak post test. N=10 larvae, 3 neuromasts/larva. Error bars=SEM.
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Image Search Results


(a) Brn3c:GFP marked hair cells (outlined) immunolabeled for phosphorylated IGF1R receptor. Scale = 10µm. (b-c) Mean pIGF1R immunofluorescence normalized to GFP immunofluorescence (b) and mean number of pIGF1R puncta (c) per hair cell cluster. *p<0.05, unpaired t test, Welch-corrected. N= 8 larvae, 1-3 neuromasts/larva. (d-f) Mean percentage of surviving hair cells 4 hours post-neomycin treatment. To calculate hair cell survival percentage, hair cell counts in neomycin treated larvae were normalized to vehicle treated larvae of same genotype. (d) Wildtype larvae treated with the IGF1R antagonist NVP-AEW541. (e-f) pappaa p170 larvae treated with SC79 (e) or recombinant IGF1 (f). * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. One-way ANOVA, Holm-Sidak post test. N=10 larvae, 3 neuromasts/larva. Error bars=SEM.

Journal: bioRxiv

Article Title: Pregnancy-associated plasma protein-aa promotes neuron survival by regulating mitochondrial function

doi: 10.1101/456632

Figure Lengend Snippet: (a) Brn3c:GFP marked hair cells (outlined) immunolabeled for phosphorylated IGF1R receptor. Scale = 10µm. (b-c) Mean pIGF1R immunofluorescence normalized to GFP immunofluorescence (b) and mean number of pIGF1R puncta (c) per hair cell cluster. *p<0.05, unpaired t test, Welch-corrected. N= 8 larvae, 1-3 neuromasts/larva. (d-f) Mean percentage of surviving hair cells 4 hours post-neomycin treatment. To calculate hair cell survival percentage, hair cell counts in neomycin treated larvae were normalized to vehicle treated larvae of same genotype. (d) Wildtype larvae treated with the IGF1R antagonist NVP-AEW541. (e-f) pappaa p170 larvae treated with SC79 (e) or recombinant IGF1 (f). * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. One-way ANOVA, Holm-Sidak post test. N=10 larvae, 3 neuromasts/larva. Error bars=SEM.

Article Snippet: Primary antibodies were as follows: phosphorylated IGF1R (anti-IGF1 receptor phospho Y1161, 1:100, rabbit IgG; Abcam), hair cells using Tg(brn3c:GFP) larvae (anti-GFP, 1:500, rabbit IgG; ThermoFisher Scientific), motor neurons using Tg(mnx1:GFP) larvae (anti-GFP, 1:500, rabbit IgG), and support cells (anti-SOX2 ab97959, 1:200, rabbit IgG; Abcam)( ).

Techniques: Immunolabeling, Immunofluorescence, Recombinant